2025, Vol. 6, Issue 2, Part D
Seroprevalence of human sapovirus IGM and IGG among children (0-5) years in some selected hospitals in Birni Kebbi, Kebbi state, Nigeria
Author(s): I Abdullahi, DN Peni, BJ Danjumma and M Yusuf
Abstract: The studies investigate the seroprevalence of human sapovirus IgG/IgM among children 0-5 years attending some selected hospitals in Kebbi State, Nigeria. Two hundred venous blood samples were collected from Argungu (35.42%), Dakingari (34.90%) and Sir Yahaya memorial hospital (29.69). Sapovirus IgG/IgM was detected using ELISA techniques and confirmed by molecular techniques using universal and specific primers set to detect the sapovirus genogroup among children. Male (51.04%) children are predominance of the respondent with high sapovirus infection 9.18%. Children within the age group 49-60 month have the highest sapovirus infection 12.50%. Children parents/guidance with secondary school level of education have 12.50% of sapovirus infection, children residing in the rural areas has 12.90% of sapovirus infection Family size from 4 and above has the highest sapovirus infection 12.90%. Parents/ guidance engaged in business activity their children are more prone to sapovirus infection (11.91%). The result showed an association among children presented with vomit 11.40%, Diarrhoea 8.00% and crèches 14.61% with sapovirus infection. Children depend on River and well water as source of drinking water have 22.73% and 7.90% of sapovirus infection respectively, Result further show that children with inclusive and exclusive mode of breast feeding had 10.26 and 17.39% of sapovirus infection. The PCR using universal primer sets detect 3 samples are sapovirus positive. The amplified sera positive were sequence and phylogenic tree was constructed using neighbouring joining method based on the relationship with members from GenBank of NCBI. Genogroup II and Genogroup IV were identifying. The study demonstrated a high seroprevalence 7.81% and GII and GIV are detected in the study area.
DOI: 10.22271/micro.2025.v6.i2d.269
Pages: 312-318 | Views: 213 | Downloads: 96
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How to cite this article:
I Abdullahi, DN Peni, BJ Danjumma, M Yusuf. Seroprevalence of human sapovirus IGM and IGG among children (0-5) years in some selected hospitals in Birni Kebbi, Kebbi state, Nigeria. J Adv Microbiol Res 2025;6(2):312-318. DOI: 10.22271/micro.2025.v6.i2d.269
