2025, Vol. 6, Issue 2, Part B
Biodiscovery of antibiotics-producing bacillus species from soil samples obtained from waste dump sites in Port Harcourt, Nigeria
Author(s): Easter Godwin Nwokah, Gloria Chile Amadi-Wali and Vivian Nkemkanma Agi
Abstract: Bacillus species have got considerable attention worldwide due to their ability to produce useful bioactive metabolites; these bioactive metabolites can be harnessed to tackle the current antimicrobial resistance the world is currently facing. The aim of this study was to evaluate antimicrobial potentials of some metabolites from Bacillus species recovered from soil samples obtained from waste dump site within Port Harcourt, Nigeria Bacillus species isolated from soil samples obtained from waste dump site were subjected to different stress condition such as time, temperature and pH for the production of bioactive metabolites. Soil samples were collected from waste dump sites and transported to laboratory for sample processing. Parent culture of Bacillus species obtained from different soil depth were prepared for the exposure study by inoculating each Bacillus species into 1000µl of peptone water in test tubes and the pH of the medium was adjusted to 4.0 and 8.0 with HCl and were subjected to temperatures of 4oC, 40oC, and 45oC and then incubated for 72hours. Already purified standard organisms were plated on Mueller Hinton agar plate and bacteria extract obtained from exposure study were dropped in the wells on the agar plate which and incubated for 24 hours at 37oC and the diameter of inhibition zones was measured in millimeter. There was no significant difference in the total heterotrophic bacteria count. Bacillus species and Staphylococcus species were consistent in the entire soil depths sampled. The result of antimicrobial susceptibility test showed that secretory molecule produced by Bacillus species isolated from 15cm soil depth were sensitive at the temperature of 40C, 400C and 450C and at pH 8.0. The bacteria extract obtained from Bacillus species in this study exhibited strong antibacterial activity against Escherichia coli, Klebsiella species, Pseudomonas species and Staphylococcus aureus as seen in the antimicrobial susceptibility test. Gas chromatography/Mass spectrometry (GC-MS) analysis conducted on the bacteria extract identified these compounds Pentadecanoic acid, 14-methyl-, methyl ester, 9-Octadecenoic acid, methyl ester, 9,17-Octadecadienal, (Z), Squalene, Pentacosanoic acid, methyl ester, 9-Octadecenal (Z), 9,12-Octadecadien-1-ol, (Z,Z)- BicycloPentylidene, 1,E-8,Z-10-Tridecatriene, 1-Hexadecyne, 1,5,9,13-Tetradecatetraene and Hexacosanoic acid, methyl ester which have been proven to exhibit antimicrobials activity against several pathogenic organisms. It was concluded that Waste dump sites has the ability to harbour Bacilli with bioactive metabolites and these bioactive metabolites produced have antimicrobial potentials.
DOI: 10.22271/micro.2025.v6.i2b.244
Pages: 117-124 | Views: 174 | Downloads: 75
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How to cite this article:
Easter Godwin Nwokah, Gloria Chile Amadi-Wali, Vivian Nkemkanma Agi. Biodiscovery of antibiotics-producing bacillus species from soil samples obtained from waste dump sites in Port Harcourt, Nigeria. J Adv Microbiol Res 2025;6(2):117-124. DOI: 10.22271/micro.2025.v6.i2b.244
