2025, Vol. 6, Issue 2, Part B

Typhoid fever also known as enteric fever is an acute systemic infection caused by Salmonella typhi. Multi-resistance of Salmonella spp to antibiotics have increased a great deal, especially in Nigeria due to indiscriminate use. The use Zinc Nanoparticles for antimicrobial activity has shown significant improvements. Multidrug resistant isolates of Salmonella typhi were determined. The genomic DNA was extracted and analysed using standard primers. Electrophoresis was performed using 16SrRNA primers. Antimicrobial activity of Zinc Oxide Nanoparticles against Salmonella typhi was determined. 42 of S. typhi isolates showed Multidrug resistance. Highest resistance was recorded in Methicillin 40(97.6%). Ciprofloxacin showed the highest sensitivity of 35(85.4%). Resistance genes were detected. Zinc Oxide showed antibacterial activity at different concentrations. With the resistance towards traditional antibiotics, fluoroquinolones are the antimicrobial agents of choice in treating MDR S. typhi. Continuous surveillance of antibiotics resistance in Salmonella typhi is recommended to understand the sources, epidemiology and transmission of MDR.