2024, Vol. 5, Issue 2, Part D

Objective: To find out Antibiotic sensitivity pattern of Staphylococcus aureus isolated from Aspirated Abscess during hospital stay of patients, OPD, Indoor, Intensive care unit (ICU) and emergency at a tertiary care teaching hospital of Janakpur.

Study design: Descriptive retrospective study

Place and duration of the study: Department of Microbiology at Madhesh Institute of Health Sciences (MIHS), Provincial Hospital Janakpur Dham from March 2024 to August 2024.

Methodology: A total of 66 Aspirated pus samples were sent for culture, and antibiotic sensitivity testing was performed. All the positive culture of 45 cases were included in this study. Patients who received antibiotics within the last 15 days or less before sample collection were excluded from the study. Also, patients under the age of 10 were not included. Isolation of microorganism was performed by a surface streak plate method on Blood agar & Selective media, Mannitol salt agar (Hi-media Pvt. Ltd. India) using sterile loops and incubated aerobically at 37 °C for 48 hours, and those cultures which becomes negative at the end of 48 hrs incubations were further incubated for 72 hrs. Antimicrobial susceptibility of isolates was tested for Staphylococcus aureus by the Kirby -bauer disc diffusion method on Muller- Hinton agar plates were used to identify the sensitivity pattern disk diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines (2023).

Results: Out of 66 samples 45 (68.18%) were positive and 21 (31.81%) were negative. A total of 66 (Male 38 & Female 28) in which samples were selected for culture and sensitivity with 28 (73.68%) male and 17(60.71%) females with age 21-30 years there were more positivity i.e.88.88% and less positivity shown in age group 61-70 years i.e. 40.0%