2024, Vol. 5, Issue 2, Part D

Objective: To find out post-operative SSI during hospital stay of patients in a generalsurgical and other surgical ward at a tertiary care teaching hospital of Janakpur.

Study design: Descriptive retrospective analysis

Place and duration of the study: Department of Microbiology at Madhesh Institute of Health Sciences (MIHS), Provincial Hospital Janakpur Dham from September 2023 to August 2024

Methodology: A total of 165 patients from different surgeries between 10 to 80 years of age were selected for the study. Isolation of microorganism was performed by a surface streak plate method on Blood agar, Mac Conkey agar, & different selective medium (Hi-media Pvt. Ltd. India) using sterile loops and incubated aerobically at 37⁰ C for 48 hours. Antimicrobial susceptibility of isolates was tested for all microorganism by the Kirby -bauer disc diffusion method on Muller- Hinton agar plates were used to identify the sensitivity pattern disk diffusion according to Clinical Laboratory Standards Institute (CLSI) guidelines (2024).

Results: Out of 66 positive cultures, Staphylococcus aureus was detected in 29(43.93) followed by Pseudomonas aeruginosa 14 (21.21), Escherichia coli 13 (19.69%), Klebsiella pneumoniae 7 (10.60%) and Acinetobacter baumannii 3 (4.54%) respectively. Of 66 positive cultures most multi drug resistant (MDR) pattern was found in Pseudomonas aeruginosa i.e. 6 (42.85%) and non-MDR pattern was in Klebsiella pneumonia 6(85.72%).