2024, Vol. 5, Issue 2, Part D
Molecular characterisation of methicillin-resistant Staphylococcus aureus associated with livestock in selected poultry farms in port-harcourt, Nigeria
Author(s): Umoh, Victor Etim, Agi, Vivian Nkemkanma, and Nwokah Easter Godwin
Abstract: Methicillin-Resistant Staphylococcus aureus (MRSA) is a strain of Gram-positive bacteria that is distinct from other Staphylococcus aureus due to its ability to develop or acquire multiple drug resistant to Beta-lactam (β-lactam) antibiotics. It is an opportunistic pathogen capable of colonizing humans, companion animals and has had increased mention in disease causation in livestock. This research was to evaluate the presence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) particularly in poultry in the Greater Port Harcourt City, Rivers State - Nigeria. A total of 195 swab (Choanal cleft and cloaca) samples were collected from 5 selected poultry farms locations in Port Harcourt namely: Elelenwo, Akpajo, Eliminiqwe, Eliozu and Umuebule – Etche. Using aseptic standard microbiological procedures, 92 (47.2%) isolates of Staphylococcus aureus were identified and these were subjected to antimicrobial susceptibility testing. Significant number of the isolates were resistant to cefoxitin and oxacillin. Out of the 92 Staphylococcus aureus isolates tested, 46 (50%) were Methicillin-Resistant Staphylococcus aureus (MRSA). About 20 representative MRSA isolates were assayed for the genes of interest (mecA and CC398 genes). Seven (7) isolates (35%) were identified as harboring the CC398 genes, signifying the prevalence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus (LA-MRSA) in Port Harcourt. It is evident that individuals who rear poultries are in higher risk of becoming LA-MRSA carriers. There is need for increased MRSA and LA-MRSA surveillance in both human and animals in our communities, in order to plan for effective control of these strain.
DOI: 10.22271/micro.2024.v5.i2d.189
Pages: 290-296 | Views: 94 | Downloads: 27
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How to cite this article:
Umoh, Victor Etim, Agi, Vivian Nkemkanma,, Nwokah Easter Godwin. Molecular characterisation of methicillin-resistant Staphylococcus aureus associated with livestock in selected poultry farms in port-harcourt, Nigeria. J Adv Microbiol Res 2024;5(2):290-296. DOI: 10.22271/micro.2024.v5.i2d.189
