2024, Vol. 5, Issue 2, Part C
Phenotypic characterization of bacteria isolated from dried crayfish (Procambarus clarkii) and fresh catfish Clarias gariepinus) in Nsukka, Nigeria
Author(s): Adonu Cyril, Ujam Treasure, Ali Ibeabuchi, Okorie Hannah, Adonu Modesta, Restus Onwusoba and Offiah Raymond
Abstract: Microorganisms in any ecological niche exhibit some characteristics that contribute to their survival in the ecosystem. A good example of such survival strategies is the production of antibiotic hydrolyzing enzymes such as ESBL and MBL enzymes in Gram negative bacteria. The study was conducted to isolate, and characterize by phenotypic methods, bacterial isolates from dried crayfish and fresh catfish sold in Nsukka, Nigeria. Isolation, characterization and antibiotic sensitivity tests were done by using standard methods. Out of the 30 randomly sampled dried crayfish in Brain-Heart infusion enrichment medium, a total of 32 bacterial isolates cultured comprised of 10 (31.3%) Klebsiella pneumonia, 5 (15.6%) Pseudomonas aeruginosa, 5 (15.6%) Serratia marcescens, 2 (6.7%) Enterobacter species, 7 (21.8%) Staphylococcus aureus and 3 (9.4%) Staphylococcus epidermidis. Similarly. 30 swab samples collected from catfish yielded a total of 21 bacteria comprising 5 (23.8%) Klebsiella pneumonia, 5 (23.8%) Enterobacter species, 3 (14.3%) Staphylococcus aureus, 3 (14.3%) Staphylococcus epidermidis, and 5 (23.8%) Escherichia coli. All the Gram negative bacteria studied were resistant to amoxicillin (R=100%), while resistance patterns varied across other antibiotics. Generally, most of the test organisms were multi-drug resistant. Of all the Gram negative bacteria isolates tested for the production of ESBL and MBL, only one strain of Klebsiella pneumoniae from crayfish was able to produce ESBL enzyme. One strain of Klebsiella pneumoniae isolated from catfish produced both ESBL and MBL whereas one strain of Escherichia coli from catfish produced ESBL only. Remedial strides such as antibiotic resistance public health education should therefore be considered and enforced so as to mitigate this globally emerging threat called antibiotic resistance.
Pages: 230-235 | Views: 689 | Downloads: 658
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How to cite this article:
Adonu Cyril, Ujam Treasure, Ali Ibeabuchi, Okorie Hannah, Adonu Modesta, Restus Onwusoba, Offiah Raymond. Phenotypic characterization of bacteria isolated from dried crayfish (Procambarus clarkii) and fresh catfish Clarias gariepinus) in Nsukka, Nigeria. J Adv Microbiol Res 2024;5(2):230-235.
