2024, Vol. 5, Issue 2, Part C
Detection and validation of highly conserved pathogen-specific genes with putative role in mycotoxin synthesis using genome-wide insilico approach
Author(s): Dr. Kanti Priya Kondala, Sravani Nidamanuri, Padmaja Musunuri, Sai Sudha Kella, Dr. K Sujatha and Chinnababu Sanapala
Abstract: The PCR-based technique was used to analyze a set of polymorphic and monomorphic DNA markers identified through experimentation on isolates of Erysiphe pisi pathogen. These putative DNA markers were used for a genome wide-search to detect the associated pathogen-specific gene sequences from the available draft sequence of Erysiphe pisi genome in public domain. The contigs in which these markers were showing homologous pairing were obtained and translated into amino-acid sequences. The sequences were analyzed for the presence of the open reading frames (ORFs) as they code for putative genes and also the domains in the sequences. The search was carried both at nucleotide and amino acid level.A significant level of correlation among the sequences with high level of similarity at nucleotide level and high level of identity at amino acid level was observed with already detected virulence genes based on multiple sequence alignment using clustalW analysis suggesting the possibility of the same function for the identified protein sequences. The predicted gene sequences were confirmed through genomic and RT-PCR testing using DNA as well as total RNA of three isolates of E. pisi in infected garden pea plant. Healthy plants served as a control for the experiments.
Pages: 201-210 | Views: 33 | Downloads: 14
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How to cite this article:
Dr. Kanti Priya Kondala, Sravani Nidamanuri, Padmaja Musunuri, Sai Sudha Kella, Dr. K Sujatha, Chinnababu Sanapala. Detection and validation of highly conserved pathogen-specific genes with putative role in mycotoxin synthesis using genome-wide insilico approach. J Adv Microbiol Res 2024;5(2):201-210.
