2024, Vol. 5, Issue 2, Part B
Isolation of bacteria from soil and water environments with phosphate solubilizing potential
Author(s): Douglas, Salome Ibietela, Nrior, Renner Renner, Igoni and Yirabari Gote
Abstract: Phosphate-solubilizing bacteria (PSB) are important in the environment due to their role in the Phosphorus cycle, making fixed phosphorus accessible to plants for their use in producing organic acids that dissolve inaccessible phosphate compounds. This study was aimed to isolate and identify bacteria with phosphate solubilizing potential from soil and aquatic environments. Rhizosphere soil samples from around leguminous plant roots and surface water samples from fresh, brackish and marine environments were collected. Using standard Microbiological procedures, these samples were inoculated on Nutrient agar and Pikoskaya’s (PVK) medium. Cultural, biochemical and molecular characterization of the isolates were done. The identified isolates were further screened for their ability to solubilize phosphate using the Phosphate Solubilizing Index (PSI). The result of the total heterotrophic bacterial counts (THBC) of the soil sample was 1.2×109 CFU/g, freshwater sample was 3.8×105 CFU/ml, 6.2×106 CFU/ml for the brackish water and 1.4×106 CFU/ml for marine sample. Total phosphate solubilizing bacterial counts in the samples were soil (4.8 x 105 CFU/g) > Brackish water (3.1x104 CFU/ml) > Marine water (8.8x103 CFU/ml) > Freshwater (3.6x103 CFU/ml). The populations of these bacteria were observed to be higher in the soil than in the three water samples. The isolates identified as PSB include Chryseobacterium aquifrigidense, Bacillus infantis, Pseudomonas xiamenensis, Bacillus flexus, and Pantoea dispersa. Results of Zone of clearance on the PVK medium and Solubilizing index (PSI) show that Pseudomonas xiamenensis is 3.15±0.06 > Bacillus flexus (2.83±0.04) > Chryseobacterium aquifrigidense (1.87±0.60) > Pantoea dispersa (1.77±0.03) > Bacillus infantis (1.43±0.02). It is therefore, recommended that farm soil with a very low soluble phosphate profile be augmented with Pseudomonas xiamenensis or Bacillus flexus to increase the soluble phosphate content, enhance proper plant growth and increase crop production.
Pages: 145-150 | Views: 163 | Downloads: 75
Download Full Article: Click Here
How to cite this article:
Douglas, Salome Ibietela, Nrior, Renner Renner, Igoni, Yirabari Gote. Isolation of bacteria from soil and water environments with phosphate solubilizing potential. J Adv Microbiol Res 2024;5(2):145-150.
