2024, Vol. 5, Issue 2, Part B
Co-occurrence of drug-resistant genes in bacteria from hospital human and environmental samples
Author(s): Okpokiri M, Okerentugba PO, Agbagwa OE and Otokunefor K
Abstract: Given the implications for antibiotic resistance and infection control, the co-occurrence of drug-resistant genes (DRGs) in bacteria is a serious concern in healthcare settings. The study aimed to investigate the co-occurrence of drug-resistant genes in bacteria associated with hospital human and environmental samples. A total of three hundred samples comprised of 150 faecal and 150 soil samples were collected in sterile containers from animal farm human and environmental samples, hospital human and environmental samples and oil-polluted soil and human samples. The bacterial isolates in the samples were analysed using standard plate count on nutrient and McConkey agar. Plasmid profiling of resistant genes was carried out using specific primers. The bacterial isolates included Pseudomonas, Serratia, Klebsiella, E. coli, Staphylococcus, Salmonella, Bacillus, Micrococcus and Alcaligenes spp. The resistant genes associated with these isolates included tetA and tetB, qnrB, blaTEM, and IncP-1genes. Most of the isolates in the environment had these genes in their plasmids while in some, the genes varied. More so, similar genes occurred amongst the isolates despite the environment indicating the presence of co-occurrence. The presence of genes coding for resistance of different classes of antibiotics implied the presence of multi-drug-resistant genes in the bacterial isolates. This could be a serious public health threat since these genes could be transferred to other isolates thereby delaying treatment of infections associated with these pathogens.
Pages: 95-100 | Views: 131 | Downloads: 93
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How to cite this article:
Okpokiri M, Okerentugba PO, Agbagwa OE, Otokunefor K. Co-occurrence of drug-resistant genes in bacteria from hospital human and environmental samples. J Adv Microbiol Res 2024;5(2):95-100.