2024, Vol. 5, Issue 2, Part A
Antibiogram of bacteria and fungi associated with surfaces of canned beverages within a tertiary institution in port Harcourt, Rivers State
Author(s): Oparaodu UA, Ogbonna SI, Chuku W, Ogbuleka NAC and Bakayesima P
Abstract: Beverage cans are exposed to various environments during production, storage and shipping during which it may be contaminated with microorganism. This study assesses the presence of microorganisms on the surface of canned beverages sold in a tertiary Institution in Port Harcourt, Rivers State. Swab samples were aseptically collected from the surfaces of canned beverages at five different retail outlets within the institution. Organisms were enumerated and identified using standard microbiological techniques. Kirby Bauer disc method was used for antibiotic sensitivity. Mean Total heterotrophic bacterial counts ranged from 1.03±0.02 to 3.8±0.14 CFU/cm2 while mean total fungal counts ranged from 1.2±0.14 to 7.5±0.7 CFU/cm2. Ten (10) Bacteria isolates identified and their percentage occurrence were; Pseudomonas sp. 1(10%), Bacillus sp. 2(20%), Enterobacter sp. 1(10%). Staphylococcus sp. 2(20%), Enterococcus sp. 1(10%), Micrococcus sp. 1(10%), Streptococcus sp. 2(20%), while seven (7) fungal isolates were; Candida albicans 2(18.2%), Trichoderma sp. 1(9.1%), Penicillium sp. 3(27.3%), Mucor sp. 3(18.2%), Aspergillus sp. 3(27.3%). Percentage occurrence from bacterial isolates showed that Streptococcus sp., Staphylococcus sp. and Bacillus sp. were the most dominant bacterial specie isolated while Asergillus sp. and Penicillium sp. were the predominant fungi isolated in all samples. Sensitivity results showed that Bacillus sp. was more susceptible to Levofloxacin, Gentamycin, Ciprofloxacin, Septrin and showed more resistance to Ampiclox, Streptomycin and Amoxil. Staphylococcus sp. was more susceptible to Levofloxacin, Chloramphenicol, Septrin, Streptomycin and showed more resistance to Gentamycin, Amoxil and Ampiclox. Enterococcus was susceptible to Ampiclox, Erythromycin, Gentamycin and resistant to Levofloxacin, Ciprofloxacin and Septrin. Pseudomonas sp. was more susceptible to Augmentin, Ciproflox, Streptomycin, Nalidixic acid and was resistant to Ampicilin, Tarivid and Gentamycin. The presence of these antibiotic resistant organisms on surfaces of canned beverages poses public health risks. Washing of canned drinks surfaces is likely to minimize the occurrence these microbes from the can surfaces and improved hygiene and handling by vendors is recommended. Usage of straws instead of drinking directly from the cans is also recommended and could minimize potential public health outbreaks.
Pages: 37-41 | Views: 201 | Downloads: 102
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How to cite this article:
Oparaodu UA, Ogbonna SI, Chuku W, Ogbuleka NAC, Bakayesima P. Antibiogram of bacteria and fungi associated with surfaces of canned beverages within a tertiary institution in port Harcourt, Rivers State. J Adv Microbiol Res 2024;5(2):37-41.
