2024, Vol. 5, Issue 2, Part A
Use of fish pond waste water in bioremediation of degreaser contaminated open farmland
Author(s): Nnemelu Ifunanya Immaculata, Janet Olufunmilayo Williams and Renner R Nrior
Abstract: Environmental pollution in the Niger Delta has been of huge concern, and bacteria have proven to be of great benefit in the degradation of petroleum hydrocarbons. A portion of Rivers State University demonstration farmland in Nkpolu-Oroworukwo, Mile 3 Diobu area of Port Harcourt, Rivers State was used for this study. Bioremediation monitoring was for a period of 56 days, analysis was carried out at an interval of 14 days: Nine (6) experimental plots were employed using a Randomized Block Design each having dimensions of 100 x 50 x 20 cm (Length x Breadth x Height) which were formed and mapped out on the agricultural soil and left fallow for 6 days before contamination on the seventh day; after which it was allowed for 21 days for proper contamination and exposure to natural environmental factors to mimic a degreaser contaminated site. Thereafter, the bio-stimulating agent (Fish pond effluent) were applied. Soil microbiological (THB, F, DUB, and DUF) and physicochemical characteristics before and after contamination (Temperature, pH, Nitrogen, potassium, phosphorus and Total hydrocarbon content) were monitored. The baseline microbiological analyses revealed that Total Heterotrophic Bacterial counts (THBC) ranged from 5.4±0.57 to 6.3±1.06 CFU/g, Degreaser Utilizing Bacteria (DUB) 2.1±0.07 to 4.1±0.64 CFU/g, Fungal counts (FC) 1.6±0.85 to 3.33±0.64 CFU/g, and Degreaser Utilizing Fungi (DUF), 1.6±0.21 to 2.5±0.35SFU/g. The results for the physicochemical parameters monitored in the duration of 56 days showed that mean pH value ranged between 5.0 to 7.38. Temperature 27.5 to 29.2oC, Nitrogen concentration 43.0 to 9.9 mg/kg, Phosphorus 0.05 to 0.58 mg/kg, Potassium 0.07 to 3.4 mg/kg and the Total Hydrocarbon Content 253 to 3983mg/kg. Bioremediation efficiency was estimated from percentage (%) reduction of Total Hydrocarbon Content (THC) from day 1 to the residual hydrocarbon at day 56 of bio-augmented/ bio-stimulation plots with the control. Results revealed amount of remediated hydrocarbon and % Bioremediation efficiency at 56 days in the different treatment plots (Initial THC contamination value of 3984 mg/kg) in a decreasing order as follows: CS+FPE (76.6%) > CS (71.5%) > CTRL 1 –US (66.4%). The study showed that bioremediation of degreaser polluted soils with combination of organic nutrients is a better degradative option. Therefore, amendment with organic nutrients like fish pond effluent is recommended for degreaser contaminated soils due to their high nutrient content as substrates for biostimulation of indigenous and augmenting biodegrading microbes.
Pages: 17-24 | Views: 267 | Downloads: 129
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How to cite this article:
Nnemelu Ifunanya Immaculata, Janet Olufunmilayo Williams, Renner R Nrior. Use of fish pond waste water in bioremediation of degreaser contaminated open farmland. J Adv Microbiol Res 2024;5(2):17-24.
