2024, Vol. 5, Issue 1, Part C
Antibiogram of bacteria isolated from oil-polluted soil in auto-mechanic workshops around a tertiary institution
Author(s): Chibuike PM, Nengi-Benwari AO, Barika PN and Robinson VK
Abstract: to investigate the antibiogram of bacteria isolated from oil-polluted soil in auto mechanic workshops around Rivers State University. Total of thirty-six (36) soil samples were collected from different locations (Maingate, Backgate and Ikwerre Road) in Port Harcourt. The samples were collected using soil auger and subjected to standard microbiological procedures such as culturing, isolation and identification. The Kirby-Bauer disc diffusion method was used to test for antibiotic susceptibility patterns. Results showed that the heterotrophic bacteria count was 1.2+0.6 × 108 for backgate, 1.3+0.2 × 108 for maingate and 2.1+0.4 × 108 for Ikwerre Road while the Hydrocarbon utilising bacteria count was 2.7+0.9 × 105 for backgate, 2.7+0.9 × 106 for maingate and 2.2+0.5 × 105 for Ikwerre Road. Microorganisms isolated from oil polluted soil include Bacillus sp, Staphylococcus sp, Micrococcus sp, Escherichia coli, Pseudomonas sp, Proteus sp, Klebsiella sp and Serratia sp. Pseudomonas sp (33.33%) had the highest frequency of occurrence while Escherichia coli (3.52%) had the least frequency of occurrence. All the bacterial isolates were resistance to Amoxicillin, Ciprofloxacin, Erythromycin, Ceftazidime, Azithromycin (100%) and sensitive to Ofloxacin, Gentamincin and Streptomycin. Antibiotic resistance in soil bacteria can be an indirect consequence of exposure to pollutants, as certain genes that confer resistance to antibiotics may also enhance tolerance to environmental stressors like oil pollutants. Antibiotic resistance is an instance of the immense capacity for natural evolution and adaptation of bacteria to diverse ecosystems. While this process seems inevitable, humans have accelerated it through various anthropogenic activities. Automobile workshops contribute to the challenge of soil contamination with petroleum hydrocarbons in addition to toxic heavy metals as a result of indiscriminate disposal of oil.
Pages: 221-226 | Views: 294 | Downloads: 104
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How to cite this article:
Chibuike PM, Nengi-Benwari AO, Barika PN, Robinson VK. Antibiogram of bacteria isolated from oil-polluted soil in auto-mechanic workshops around a tertiary institution. J Adv Microbiol Res 2024;5(1):221-226.
