2024, Vol. 5, Issue 1, Part C
Response of Escherichia coli isolated from Tympanotonos fuscatus and Pachymelania aurita to conventional antibiotics, Piper guineense and seed extracts
Author(s): Williams JO, Sampson T and Deeyah FE
Abstract: Seafoods like periwinkles are reported to contain high bacterial load and serve as a route of ingesting pathogenic E. coli into human body as a result of poor sanitary conditions of the open markets and indiscriminate disposal of sewage into its water habitat. This study was carried out to investigate the Response of Escherichia coli Isolated from Periwinkles (Tympanotonos fuscatus and Pachymelania aurita) to Conventional Antibiotics and Uziza (Piper guineense) Leaf and Seed Extracts. Fifty-four (54) samples (raw and parboiled) were purchased from three different markets for three months using standard methods. Antibiotics disk diffusion method was carried out as well as extracts from Uziza leaf and seed was assayed using agar well and agar dilution methods. The susceptibility patterns of E. coli to antibiotics showed that all the isolates (100%) were susceptible to Levofloxacin and ofloxacin and a decreasing trend of resistance in the order; Ampiclox and Cefotaxime (89%) > Imipenem and Nalidixic acid (78%) >Amoxicillin- clavulanate (66.7%) > Cefexime (56%) > ceftriaxone-Sulbactam (33.3%) >Gentamycin (22%) > Cefuroxime (11%). The antimicrobial activity of (Uziza) leaf showed that at higher concentrations (300mg/ml) all the isolates were inhibited. While the minimum inhibitory concentration (MIC) observed for the leaf extract was 37.5mg/ml, the MIC of the seed was 9.375mg/ml. Molecular studies confirmed all the isolates screened had the blaTEM gene present in their genome. Out of the twenty-seven (27) E. coli isolates, (88.8%) had multidrug resistance index ≥ 0.2. The study therefore showed that the seed extract showed more potency than the leaf extract. Uziza leaf and seeds could therefore be used in the control of E. coli associated with sea food, while discouraging indiscriminate use of antibiotics.
Pages: 213-220 | Views: 236 | Downloads: 128
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How to cite this article:
Williams JO, Sampson T, Deeyah FE. Response of Escherichia coli isolated from Tympanotonos fuscatus and Pachymelania aurita to conventional antibiotics, Piper guineense and seed extracts. J Adv Microbiol Res 2024;5(1):213-220.