2024, Vol. 5, Issue 1, Part C
Isolation and identification of Aspergillus niger from the onions in Bharuch, Gujarat, India
Author(s): Dr. Mahendra Pal
Abstract: A mycological study was undertaken to know the natural occurrence of Aspergillus niger, a saprobic filamentous fungus, on the raw onions. A total of 47 onions collected from vegetable markets and street vendors were examined by swab technique. The sterile cotton swab dipped in glucose solution and chloramphenicol solution was rubbed on the bulb of each onion, and streaked on to the plates of Pal sunflower seed medium (Pal medium) and APRM agar. The inoculated plates were incubated at 30 0 C, and examined daily for the fungal growth. The fungal growth resembling Aspergillus was observed in 21 out of 47 onion samples. The cultural growth and detailed microscopic morphology in Narayan stain confirmed the identity of all the 15 fungal isolates as Aspergillus niger. Since A. niger has the potential to infect the humans, the housewives and others who work in kitchen should take great precautions to avoid the transmission of this medically important fungus. To the authors knowledge, this seems to be first study on the isolation of A. niger from onions on APRM agar and Pal medium.
Pages: 178-180 | Views: 906 | Downloads: 643
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How to cite this article:
Dr. Mahendra Pal. Isolation and identification of Aspergillus niger from the onions in Bharuch, Gujarat, India. J Adv Microbiol Res 2024;5(1):178-180.
