2023, Vol. 4, Issue 2, Part A
The effect of ketogenic diet on gut microbiota of Albino Rat
Author(s): Bull Ibiso, Agi Nkemkanma Vivian, Rhoda ME Nwalozie and Ollor Amba Ollor
Abstract: The human gastrointestinal tract has the largest number of microorganisms including bacteria, archaea and eukaryotes as compared to other areas of the body. The collection of these microorganisms colonising the gastrointestinal tract is termed the gut microbiota. The human microbial community is strictly influenced by diet, and a good gut microbial community is connected with a better health. The ketogenic diet is a form of diet focusing on reduced intake of carbohydrates and increased intake of fat. Itt has gained popularity as a method to reduce weight and this pattern, by reducing certain type of food, may affect the gut microbiota composition and its related influence on host physiology This study evaluated the effect of ketogenic diet on the gut microbiota of albino rat model. A total of 25 rats were used and divided into 5 groups having 5 rats each. After acclimatization for 2 weeks, group (2, 3, 4 and 5) were fed with ketogenic diet prepared by mixing 65% of standard feed with 35% of commercial butter for 7 and 14 days respectively and their weights recorded. Group 1 rats served as the control group and were fed with pellet and water only throughout the experiment. Stool samples were collected aseptically by pulling from each group into sterile stool sample bottles and sent to the laboratory for immediate culture immediately after acclamitzation, at the end of 7 days and at the end of 14 days respectively. They were analysed using standard convectional microbiological method, moecular ananlysis, total heterotrophic plate count and antibiotic susceptibility were also done. The statistical tool used for analysis was analysis of variance and chi square with p< 0.05. The results after measuring the body weight of the rats after acclimatization, 7 days and 14 days for all the groups showed that the body weights of group 1 rats were relatively stable and there was no significant difference. There was weight loss in the groups that were fed with ketogenic diet, with significant difference in group 2 and 3 but no significant difference in group 4 and 5 following a cut off of p< 0.05. The heterotrophic plate count of bacteria revealed a decrease in the number of colonies in the groups that were fed with ketogenic diet while the count in group 1 (control) were relatively stable. The bacterial count in all the groups after acclimatization was higher than the count in the groups (2, 3, 4 and 5) that were fed with high fat. Hence, there is decrease in count. It was also observed that the rats that were fed with ketogenic diet had reduced stool mass. The rate of resistance to the antibiotics used for susceptibility testing was higher with Bacillus spp and Klebsiella spp. The molecular technique proved to be more specific and accurate than the convectional technique in identification and characterization of the isolates. As seen from this study, ketogenic diet affirms weight loss and altered the bacterial count.
Pages: 60-66 | Views: 107 | Downloads: 37
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How to cite this article:
Bull Ibiso, Agi Nkemkanma Vivian, Rhoda ME Nwalozie, Ollor Amba Ollor. The effect of ketogenic diet on gut microbiota of Albino Rat. J Adv Microbiol Res 2023;4(2):60-66.