2023, Vol. 4, Issue 1, Part B
Morphological and biochemical characterization of oil degrading isolated microorganisms
Author(s): Rajendra Pavan and Brijesh Sharma
Abstract: Environmental microbiology's most crucial component is the effective bioremediation of dangerous and deadly contaminants. The biological procedure known as "bioremediation" uses living microorganisms to eliminate pollutants or toxins from soil or water. Microbes are used to detoxify and degrade environmental pollutants in this process. Toxic hydrocarbons are converted into innocuous compounds including organic acids, aldehydes, and eventually carbon dioxide and water by this process. The ecology is impacted by oil contamination, which in turn influences the bioremediation procedures. Different hydrocarbon groups in a complex mixture have varying bioavailability and biodegradability, which results in varying intrinsic bioremediation application. Therefore, before conducting remedial action, a site assessment should determine the hydrocarbon composition based on the source of the pollution. This study's site assessment yields a number of broad conclusions that might be used to build degradation strategies. In comparison to non-contaminated soil, contaminated locations have a relatively high concentration of microorganisms that break down hydrocarbons. However, the degrader numbers differ between locations and most likely change in response to the length of pollutant exposure. According to the findings of the current study, soil samples contaminated with petroleum were taken from a variety of oil-affected locations. The physical and chemical characteristics of the soil samples were ascertained. There was a direct correlation between contamination levels and carbon content. Additionally, it was discovered that the soil samples were lacking in phosphorus and nitrogen. The moisture content and water retention capacity of soil samples were low while the electric conductivity was high. The altered physicochemical characteristics of the contaminated soils render them unsuitable for the development of agricultural crops as well as the typical soil flora. By using the enrichment culture technique in selective minimum media (Bushnell-Hass) with the 2T engine as the only source of carbon, a total of eleven bacterial isolates and one fungus isolate were obtained.
Pages: 96-102 | Views: 39 | Downloads: 27
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How to cite this article:
Rajendra Pavan, Brijesh Sharma. Morphological and biochemical characterization of oil degrading isolated microorganisms. J Adv Microbiol Res 2023;4(1):96-102.