Journal of Advances in Microbiology Research
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P-ISSN: 2709-9431, E-ISSN: 2709-944X

2023, Vol. 4, Issue 1, Part A


Formulation and cultivation of microorganism using alternative media from agricultural wastes


Author(s): Patience O Adomi and Obaro L Oyubu

Abstract: The research was designed to formulate culture media with agro wastes, culture bacteria and fungi therein and to determine the proximate and phytochemicals of the agricultural wastes. Yam peels, pawpaw peels, plantain peels and egg shell were collected from vendors, washed and sundried for 2-3 weeks then ground into powder. Specific quantities of samples were prepared and mixed with agar agar. The formulated media were inoculated with bacteria and fungi. Results indicated that formulated media supported the growth of microorganisms. The proximate and mineral composition analyses of the media included ash content of 5.94% for pawpaw peel, 3.98% (plantain peel), 4.83% (egg shell) and 4.97% (yam peels). Moisture content for the various peels were 3.98%. 2.98% 1.87% and 3.67% respectively for yam peels, plantain peels, pawpaw peel and egg shell. Carbohydrates, proteins, fats, minerals and vitamin C were shown to be present in formulated media. Phytochemicals results/anti nutrients (in mg/100g) showed that yam peel contained the following; alkaloids (3.50), saponins (2.87), flavonoids (2.70), tannins (41.05), oxalates (2.98), steroids (2.30) and reducing sugar. Plantain peels contained alkaloids (3.20), saponin (2.20), flavonoids (2.0), cardiac glycoside, phytate and reducing sugar (2.98) respectively. Egg shell phytochemical composition included alkaloids (1.2), saponins (1.86), flavonoids (1.6), and oxalates (0.82). Formulated media from agricultural waste supported the growth of bacteria and fungi and could be useful for microbial culture for diagnostic and research purposes.

Pages: 26-31 | Views: 15 | Downloads: 13

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How to cite this article:
Patience O Adomi, Obaro L Oyubu. Formulation and cultivation of microorganism using alternative media from agricultural wastes. J Adv Microbiol Res 2023;4(1):26-31.
Journal of Advances in Microbiology Research
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