Journal of Advances in Microbiology Research
2023, Vol. 4, Issue 1, Part A
Changes in Trichomonas vaginalis IST1- gene after metronidazole therapy
Author(s): Aseel Isam Alnuri
Abstract: Background: Virginal trichomoniasis is the most prevalent sexually transmitted illness with clinical manifestations vary from asymptomatic to symptomatic, including severe urethritis, vulvo-vaginitis, and cervicitis. Internal transcribed spacers (ITS1) are non-functional ribosomal RNA snippets that transcribed without further translation. This gene is critical in determining whether metronidazole should be used in treatment or not.Aims: This research was intended for the first time in Iraq to discover the copy number changes in the T. vaginalis ITS1 gene after metronidazole using molecular qPCR.Methods: Fifty samples were obtained metronidazole sensitive group and resistant-group who were referred to the Gynecology Department of Al-Khansaa Teaching Hospital in Mosul, Iraq. All samples were examined wet mount and grown in 50 ml of TYM media. Metronidazole Inhibition percentage assay using Meingassner method. The ITS1 fragment was amplified using qPCR to study the changes in this fragment expression in both groups.Results: The results revealed that the resistance samples showed significant lower inhibition zone in comparison to sensitive group. Significant increase in IST1 sequence copy number in to metronidazole-resistance group.Conclusion: ITS1 copy number may serve as an indicator for identifying resistance, which might lead to the development of management and treatment techniques to limit its spread.
Pages: 16-19 | Views: 24 | Downloads: 13
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How to cite this article:
Aseel Isam Alnuri. Changes in Trichomonas vaginalis IST1- gene after metronidazole therapy. J Adv Microbiol Res 2023;4(1):16-19.