2022, Vol. 3, Issue 1, Part A
Preliminary studies into transmogrification of Aspergillus genome for over expression of cellulase
Author(s): Emoleila Ejiya Itoandon, Femi Adams, Salau Adetola Moisat and Azeez Tijani
Abstract: The aim of this investigation was achieved by exposing native Aspergillus niger (WldAN) to an ultraviolet (UV) germicidal lamp (256 nm) kept at a distance of 40 cm for a time interval ranging from 2 –14 minutes, in a total of 56 minutes and finally using the improved strain to synthesize a more stable cellulase. The results showed growth improvement of the modified isolate with a density of 3.54 g/ml at the 9th hour as compared to 3.36 g/ml density of the native strain at the 7th hour on the 3rd day. Cellulase produced by submerged fermentation at 25 oC for 4 days using the native Aspergillus niger (WldAn) and modulated Aspergillus niger (UVAn) respectively recorded activities of 3.50 U/ml at 60 oC and 2.85 U/ml at pH 4.0 as compared to 2.40 U/ml at 50 oC and 2.24 U/ml at pH 5.0 recorded for cellulase synthase from the native strain
DOI: 10.22271/micro.2022.v3.i1a.18
Pages: 01-06 | Views: 1822 | Downloads: 1025
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How to cite this article:
Emoleila Ejiya Itoandon, Femi Adams, Salau Adetola Moisat, Azeez Tijani. Preliminary studies into transmogrification of Aspergillus genome for over expression of cellulase. J Adv Microbiol Res 2022;3(1):01-06. DOI: 10.22271/micro.2022.v3.i1a.18
