2021, Vol. 2, Issue 2, Part A
Laboratory techniques for isolation, identification and molecular detection of Newcastle disease virus: A systematic review
Author(s): Mahendra Pal, Sagni Ragassa, Morka Dandecha and Kirubel Paulos Gutama
Abstract: Newcastle disease virus is a RNA virus that is responsible for causing Newcastle disease, which primarily affects various bird species. The virus has the potential to spread to animals since it can cause conjunctivitis in people who work with poultry. The disease has a significant negative economic impact on the world's poultry production. Cell culture and chicken embryonated eggs are utilized in the viral isolation process. The virus can be identified from blood or allantoic fluids using a variety of techniques, including hemagglutination, hemagglutination inhibition, enzyme-linked immunosorbent assay, and neutralization testing. The mean death time, intravenous, and intracerebral pathogenicity indices can be used to identify viral pathotypes. Reverse transcription-polymerase chain reaction, gene sequencing, and phylogenetic tree analysis are contemporary approaches used to identify and characterize the virus genotypes at the molecular level. In conclusion, this review describes how to isolate and identify the virus that causes Newcastle disease, as well as how to check for the virus's molecular existence, identify its pathotypes, and analyze its genotype. Therefore, using embryonated chicken eggs is generally advised for virus isolation, and further serological and molecular testing should be performed to establish the presence of the virus in the allantoic fluid.
Pages: 53-58 | Views: 206 | Downloads: 74
Download Full Article: Click Here
How to cite this article:
Mahendra Pal, Sagni Ragassa, Morka Dandecha, Kirubel Paulos Gutama. Laboratory techniques for isolation, identification and molecular detection of Newcastle disease virus: A systematic review. J Adv Microbiol Res 2021;2(2):53-58.
