2021, Vol. 2, Issue 2, Part A
Genetic diversity of plant growth promoting rhizobial and non-rhizobial isolates from green gram (Vigna radiata L.) based on Amplified ribosomal DNA restriction analysis
Author(s): Archana Dhole and Harsha Shelat
Abstract: The increasing awareness about organic farming in farming community of India has stimulated the isolation and identification of large number of rhizobia strains from the root nodules and directed to studies on their diversity in the nodule niche. The bio-fertilizer inoculants containing diverse strains of rhizobia have been developed to improve soil fertility and increase symbiotic nitrogen fixation in the legumes. In spite of this success, there is paucity of data on diversity and genetic variation of rhizobia and associated genera present in root nodules as well as interaction between them. So this study aims to isolate and find out the genetic diversity of rhizobial and non rhizobial strains from root nodules of green gram. Comparisons of communities and variations in their DNA sequences existing in PCR-amplified 16s rRNA genes were studied by Amplified ribosomal DNA restriction analysis (ARDRA). The amplified PCR product from the 15 Rhizobial isolates and 38 non rhizobial isolates obtained from green gram root nodules were digested by tetra cutter restriction endonucleases (Hae III and Hinf I). ARDRA profile specified that the structure of community of rhizobial type and non rhizobial isolates was diverse and variable since four different clusters were observed separately by rhizobial type and non rhizobial isolates. Furthermore, 17 isolates showed phosphate solubilization ability within 5 days and production of IAA was varied greatly among both rhizobial type and NRE isolates ranging from 116. 50 µg ml-1 to 10.50 µg ml-1. Therefore, the results of this study certainly suggests that the rhizobial and NRE isolates have great diversity and variability inside the nodule environment which help the plant growth, development and proliferation due to phosphate solubilization and IAA production. Thus, these isolates as a consortium may be explored as bio-inoculants for sustainable agriculture.
Pages: 33-45 | Views: 290 | Downloads: 131
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How to cite this article:
Archana Dhole, Harsha Shelat. Genetic diversity of plant growth promoting rhizobial and non-rhizobial isolates from green gram (Vigna radiata L.) based on Amplified ribosomal DNA restriction analysis. J Adv Microbiol Res 2021;2(2):33-45.