Journal of Advances in Microbiology Research
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P-ISSN: 2709-9431, E-ISSN: 2709-944X

2021, Vol. 2, Issue 2, Part A


Characterization of cellulolytic actinobacteria from waste material of sugar factories in the South Gujarat and Maharashtra region


Author(s): Rakholiya AK, Bhimani AA and Bhimani HD

Abstract: This study investigates the isolation, screening and characterization of cellulolytic actinobacteria from sugarcane press mud samples of sugar factories in South Gujarat. Heat and CaCO3 treatment enriched actinomycetes population. Out of 52 isolates, MSF9 and BSF22 exhibited significant CMCase production. Morphological, cultural, biochemical and molecular characteristics were examined. Both strains were citrate, indole, and urease positive. MSF9 was positive for sucrose and lactose, while both strains showed glucose and maltose positivity. Molecular analysis identified MSF9 as S. celluloflavus and BSF22 as S. longisporoflavus. Optimal conditions for cellulase activity and protein production were explored. Results revealed that both strains displayed maximum cellulase (CMCase) activity and total protein production at 45 °C. MSF9 exhibited the highest enzyme activity (1.89 U ml-1) and protein production (2.42 mg ml-1), while BSF22 showed significant activity (1.86 U ml-1) and protein production (1.79 mg ml-1) 45 °C temperature. Enzyme activity and protein production were lowest at 25 °C. The impact of pH on enzyme activity and protein production was studied, both strains showing peak CMCase activity at pH 7 (MSF9: 0.75 U ml-1; BSF22: 0.93 U ml-1) and lowest activity at pH 4. Varying carboxymethylcellulose (CMC) concentrations (0.5% to 3.0%) influenced enzyme activity and protein production, with optimal values at 2% CMC (MSF9: 1.38 U ml-1, 3.18 mg ml-1; BSF22: 1.41 U ml-1, 3.53 mg ml-1).

Pages: 59-65 | Views: 151 | Downloads: 59

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Journal of Advances in Microbiology Research
How to cite this article:
Rakholiya AK, Bhimani AA, Bhimani HD. Characterization of cellulolytic actinobacteria from waste material of sugar factories in the South Gujarat and Maharashtra region. J Adv Microbiol Res 2021;2(2):59-65.
Journal of Advances in Microbiology Research
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