Journal of Advances in Microbiology Research
2021, Vol. 2, Issue 2, Part A
Camel viral disease outbreak investigation on Negele Borena zones of Oromia regional state of Ethiopia
Author(s): Aregitu Mekuriaw Arega and Legesse Bekele W Mariam
Abstract: Camel is an important domestic animal uniquely adapted to the hot and arid environment, but its value to Ethiopian pastoralists is unbalanced to its resource potential due to the presence of various infectious diseases in the area. Therefore this study was conducted to isolate and characterize the emerging suspected case samples collected in camel rearing areas in Borena Zone of Ethiopia in 2007at national veterinary institute (NVI) from December 2010 to April 2011. A total of eleven Negele Borena camel postmortem tissue samples (four lungs, four liver, two heart, and one heart blood) were processed by using cell culture techniques,AGID test and molecular techniques. The suspected viral pathogen was isolated from all of the samples processed and inoculated on Vero cell cultures with a variable amount of detectable CPE and growth character within 5 to 8 days. The supernatant samples exhibiting CPE were taken and tested for presence of both DNA and RNA viruses, using universal degenerate oligonucleo prim– polymerase chain reaction (DOP-PCR) and conventional polymerase chain reaction (PCR) techniques. All of the samples tested by DOP-PCR were positive for presence of RNA virus, but negative for DNA virus and Peste Des Petits Ruminants Virus (PPRV). Furthermore the immune diffusion test result on an isolated virus conducted by using known PPR antibodies was negative. Therefore, the actual virus in this study was ruled out that the etiological agent/s of sudden camel mortality occurred in 2007 in Borena Zone of Ethiopia were an RNA virus and also it was not a member of the genus PPR virus that are detectable by existing primers. On the bases of this remark, the viral causative agent of the disease was further molecularly characterized by using specific primed PCR, optimization of techniques and sequencing machines.
Pages: 14-19 | Views: 478 | Downloads: 290
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How to cite this article:
Aregitu Mekuriaw Arega, Legesse Bekele W Mariam. Camel viral disease outbreak investigation on Negele Borena zones of Oromia regional state of Ethiopia. J Adv Microbiol Res 2021;2(2):14-19.