2021, Vol. 2, Issue 2, Part A
Surface spreading sporulation (SSS) technique for microfungal purification
Author(s): Emoleila Itoandon, Femi Adams and Tijani Azeez
Abstract: A technique was used for the purpose of separating microscopic filamentous molds. The primary aim was to establish a method that will conveniently distribute vegetative spores across the surface of a culture medium allowing easy detection of desired strain. A contaminated Sabouraud Dextrose Agar (SDA) cultured plate previously used for isolation at 25 oC after 5 days was used for the purpose of this investigation. The culture plate showed a mixture of probable isolates after incubation period signifying a case of cross contamination. Following standard laboratory procedure, the following isolates: Aspergillus Niger, Aspergillus terreus, Penicillium funiculosum and Penicillium chrysogenum were recovered using the surface spreading technique. The investigation confirmed the level of microbial co-existence which is often difficult to manage in cases of pollution caused by molds due to their growth pattern. On the other hand, the practise provided a simple and effective application in handling purification of multicellular sporulation.
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How to cite this article:
Emoleila Itoandon, Femi Adams, Tijani Azeez. Surface spreading sporulation (SSS) technique for microfungal purification. J Adv Microbiol Res 2021;2(2):01-04. DOI: 10.22271/micro.2021.v2.i2a.13