2021, Vol. 2, Issue 1, Part A
Bioactive metabolites from ethyl acetate extract of leaves of Melia dubia L., against human and plant microbial pathogens
Author(s): JP Nikkitha, P Suresh, S Kameshwaran, M Rekha, G Mahendra Perumal and V Shanmugaiah
Abstract: Antimicrobial metabolites are screening from medicinal plants is a promising technique to combat the growing issue of drug-resistant infections in humans and plants. In the present study, antimicrobial activity of Melia dubia L., was tested against human and plant pathogenic microbes by well diffusion method. Among three solvent system, ethyl acetate extract of M. dubia was showed significant activity towards human and plant pathogens such as Escherichia coli MTCC443, Salmonella typhi MTCC733, Bacillus cereus MTCC 430, Staphylococcus epidermidis MTCC 10623, Klebseilla sp. MTCC 3384, Rhizoctonia solani, Macrophomina phaseolina, Sclerotium rolfsii and Fusarium oxysporum with different level of zone of inhibition (ZOI) from 2 mm to 15 mm and 5 mm to 27 compared to control. The maximum quantity of phytochemical constituents like tannins, total phenol, flavonoids, saponins and alkaloids were obtained from ethyl acetate extract of M. dubia in comparison with other two solvent system such as acetone and petroleum ether. From ethyl acetate extracts of M. dubia, four spots of biomolecules with Rf values of 0.46, 0.41, 0.32 and 0.18 were detected on thin layer chromatography (TLC). Bioactive compounds are now undergoing purification and characterisation from M. dubia.
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How to cite this article:
JP Nikkitha, P Suresh, S Kameshwaran, M Rekha, G Mahendra Perumal, V Shanmugaiah. Bioactive metabolites from ethyl acetate extract of leaves of Melia dubia L., against human and plant microbial pathogens. J Adv Microbiol Res 2021;2(1):13-21.