2020, Vol. 1, Issue 2, Part A
Toxicity of spent automobile battery on Nitrobacter and nitrosomonas species in soil
Author(s): Nrior Renner Renner, Awari Victoria Ginika and Ajikwaru Glory Praisejah
Abstract: Aim: To evaluate the concentration of spent automobile battery that could cause potential toxic effect on the microorganisms: Nitrobacter sp. and Nitrosomonas sp. in soil.Study Design: The study employs experimental design, statistical analysis of the data and interpretation.Place and Duration of Study: Soil sample were collected from Agricultural, Research and Development Farm of Rivers state University, Port Harcourt, Nigeria. Spent automobile batteries were obtained from the mechanic workshop at Ikoku, Mile 1, Port Harcourt, Rivers state. These samples were transported in ice pack to the Microbiology Laboratory of Rivers State University, Port Harcourt, Nigeria, within 24 hours for analyses. Methodology: Toxicity test procedures were carried out by submerging ten grams of automobile battery into sterile transparent plastic rubber containing 1000g of soil samples. Toxicity test was carried out using six plastic rubbers containing automobile acid battery + soil (set-up samples). Each set-up sample containing different toxicant concentration (0%, 6.5%, 12.5%, 25%, 50% and 75%) was inoculated with 1ml of test organism using spread plate technique on Winogradsky media. The cultures were incubated at 35°C for 18 to 24 hours. Thereafter, plates were counted at interval of 0hr, 4hrs, 8hrs, 12hrs and 24hrs. Median lethal concentration (LC50) was determined using SPSS version 20.Results: The results showed increase in percentage logarithm mortality of Nitrosomonas species with increased toxicant concentration and exposure time. The media lethal concentration (LC50) of the spent motor battery used decreased in the following order; spent automobile battery in soil environment + Nitrosomonas (36.80%) > automobile battery in Nitrobacter (47.49%). However, automobile battery in Nitrosomonas had lower toxicity than the Nitrobacter.Conclusion: The result revealed that hazardous chemical from battery can cause environmental hazard which affect microorganisms in soil. Hence, Proper disposal of automobile spent battery should be practiced to avoid environmental pollution that can be detrimental to plant and human health.
Pages: 51-60 | Views: 182 | Downloads: 73
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How to cite this article:
Nrior Renner Renner, Awari Victoria Ginika, Ajikwaru Glory Praisejah. Toxicity of spent automobile battery on Nitrobacter and nitrosomonas species in soil. J Adv Microbiol Res 2020;1(2):51-60.