2020, Vol. 1, Issue 2, Part A
Hydrocarbonoclastic bacteria and organic nutrients mixture potential for biodegradation of hydrocarbon contaminated soils in Niger Delta
Author(s): Victoria Ginika Awari, David Nwagozie Ogbonna, Akani Nnedie Patience and Douglas Salome I
Abstract: Biodegradation, the use of hydrocarbon degrading microbes also known as hydrocarbonoclastic microbes and/or the use of nutrient organics to clean up crude oil hydrocarbon contaminated soils by assessment of the potentials of the bacteria; Comamonas testosteroni and nutrient organics; goat manure (GM), fish waste (FW) were investigated. The aim is to adopt it as treatment approach in biodegradation of crude oil contaminated soils in Niger Delta regions. A total of ten experimental setups of 5000g soil in each, where; a group contained 5% crude oil (5% HCS) and another group contained 10% crude oil (10%HCS) were laid out in an experimental plot for a period of 3 weeks. Applications of treatments (CM, GF and FS) were carried out. Baseline characteristics of soils were determined. Soil samples were collected at seven days interval to monitor microbiological parameters including; total heterotrophic bacteria and fungi, hydrocarbon utilizing bacteria and fungi and physiochemical parameters including; nitrate, sulphate, potassium, magnesium, pH, temperature and total petroleum hydrocarbon. The values of the bioremediation efficiency were determined by the differences in the percentage of TPH concentrations on initial day and on the final day of experiment. The results of the experiments were statistically analyzed. The TPH concentration value on day 1 recorded; 10328.03mg/kg. This reduced in the different treatments employed as degradation progressed and revealed percentage of TPH concentrations in increasing order accordingly: Ctrl (125.71 mg/kg; 1.21%) < CM (2261.01 mg/kg; 21.89%) < GM+CM (7313.47 mg/kg; 70.81%) < FW+CM (7614.74 mg/kg; 1.21%) < GM+FW+CM (8100.59 mg/kg; 78.43%). 5% CS followed similar progression. This result showed that the nutrient organics enhanced the biodegradiation capability of the hydrocarbon degrading bacteria. Although, biodegradation using combination treatment of the nutrient organics added to the bacteria proved more effective and achieved a greater percentage of TPH biodegraded. It is therefore recommended as effective biodegradation option in cleaning up of hydrocarbon contaminated soils in Niger Delta.
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How to cite this article:
Victoria Ginika Awari, David Nwagozie Ogbonna, Akani Nnedie Patience, Douglas Salome I. Hydrocarbonoclastic bacteria and organic nutrients mixture potential for biodegradation of hydrocarbon contaminated soils in Niger Delta. J Adv Microbiol Res 2020;1(2):06-20.