2020, Vol. 1, Issue 2, Part A
Impactive role of reactive chlorine in chlorinated water and the survival of selected rhizobia strains commonly used as inoculant
Author(s): Ajayi OO, Amao TO, Bitire T, Dianda M and Fagade OE
Abstract: Chlorination is a commonly used method for the disinfection of water in many homes and farms. The use of chlorinated water is believed to kill or affect rhizobia negatively, this study was carried out to determine the effect of different concentrations of NaCl and chlorine powder (100 mg/L- 300 mg/L) on the growth of selected Rhizobia strains including USDA110, USDA136, 532C, RACA6 and FA3 which are Bradyrhizobium japonicum strains commonly used for soybean. They were inoculated on chlorine induced Congo-red medium and incubated for 10days and growth was studied from the 3rd day. The growth of the strains were unaffected by NaCl, FA3 and RACA6 showed no growth at 150mg/L on the 3rd day but after 7 days RACA6 showed signs of growth at 150 mg/L. the growth of USDA110 and 532C was affected at 250 mg/L and 300 mg/L respectively from the 3rd day till the 10th day. USDA136 was unaffected even at 300 mg/L from the 3rd day through the 10th day. Chlorination had effect on the growth of the strains particularly strain FA3 and RACA6, therefore it is highly important to monitor the amount of chlorine in tap water that will be used in watering soybean plants particularly those inoculated with these strains.
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How to cite this article:
Ajayi OO, Amao TO, Bitire T, Dianda M, Fagade OE. Impactive role of reactive chlorine in chlorinated water and the survival of selected rhizobia strains commonly used as inoculant. J Adv Microbiol Res 2020;1(2):01-05.