2020, Vol. 1, Issue 2, Part A
Isolation and probiotic characterization of lactic acid bacteria from traditional milk and identification of Lactobacillus sp. by PCR method
Author(s): Joseph Antony Reneese P, Ragavi M and Sankar R
Abstract: Probiotic microbiota plays a vital role in gastrointestinal health and possesses other beneficial attributes such as antimicrobial and antibiotic agents. In this study, A total of 10 traditional milk samples were collected from the Southern regions of Tamil Nadu, India. 55 bacterial isolates were isolated based on their sustainability in the gut environment and based on their antagonistic and antioxidant capacity. A series of established in vitro tests were conducted to determine the probiotic potential of isolates. These tests include bile salt tolerance, antibiotic resistance, ability to survive at a lower pH range. From the results 9 bacterial strains BM 3, BM 19, CU 27, CU 29, CU31, CU 40, TG 18, TM 6 and TM 37 showed good antagonistic and antioxidant activity than other strains. Lactobacillus bacteria were identified to genus level by using Lactobacillus specific primers and detected by PCR analysis. Out of these 55 beneficial strains 38 bacterial strains were identified as Lactic acid bacteria and come under the genus Lactobacillus.
Pages: 75-81 | Views: 186 | Downloads: 73
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How to cite this article:
Joseph Antony Reneese P, Ragavi M, Sankar R. Isolation and probiotic characterization of lactic acid bacteria from traditional milk and identification of Lactobacillus sp. by PCR method. J Adv Microbiol Res 2020;1(2):75-81.
