2020, Vol. 1, Issue 1, Part A
Antimicrobial activities of green algae on microbial isolates
Author(s): Otolorin Isaac G, Osuala Oluchi J, Ezemba Chinyere C, Ezemba Arinze S and Etikudike Victor O
Abstract: Algae are a group of organism that contain photosynthetic pigments but are not plants. They can appear in colonies that are very visible especially in the environment. They have been previously reported to have economical importance as a source of crude oil and as sources of food and a number of pharmaceutical and industrial products for humans. Several authors studied the antimicrobial activities of marine algae in different parts of the world. The aim of this study is to evaluate the antimicrobial activity and bioactive compounds of ethanol and n-hexane extracts of green algae from the environment on some microbial isolate. The Green algae was collected from the walls of the hostels and the active compounds were extracted using maceration in ethanol and nonpolar compounds were extracted using soxhlet extraction with n-hexane. The extracts were tested on certain microbial isolates which includes Escheichia coli, Salmonella sp, Staphylococcus aureus, Bacillus sp and Candida sp. From the results, on the E. coli, the highest activity of the extract was found at 400mg/ml. For the Bacillus sp, the highest activity of the extract was found at 50mg/ml with 9mm. The Salmonella sp inhibited at 50mg/ml. S.aureus, showed same mean zone of inhibition (MZI) for 400mg/ml, 200mg/ml and 100mg/ml at 1.50mm. Inhibition of the Candida sp was highest at 50mg/ml with 7.50mm. Compounds identified from the extracts includes Cyclotrisiloxane, hexamethyl- (22.71%), N-Methyl-1-adamantaneacetamide (9.06%), Pentasiloxane, dodecamethyl- (2.03%), Cyclohexasiloxane, dodecamethyl(4.37%), Caffeine (13.36%), Acridin-9-yl-(4-trifluoromethoxy-phenyl)-amine (11.04%), Glycine, N-[4-[(trimethylsilyl)oxybenzoyl]-, methyl ester (1.49%). The result shows that the extracts showed antimicrobial properties on the isolates tested due to certain compounds identified.
Pages: 37-43 | Views: 162 | Downloads: 54
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How to cite this article:
Otolorin Isaac G, Osuala Oluchi J, Ezemba Chinyere C, Ezemba Arinze S, Etikudike Victor O. Antimicrobial activities of green algae on microbial isolates. J Adv Microbiol Res 2020;1(1):37-43.